BIODEGRADATION STUDY OF PYCNOPORUS SANGUINEUS AND ITS EFFECTS ON STRUCTURAL AND CHEMICAL FEATURES ON OIL PALM BIOMASS CHIPS.

Pooja Singh, Othman Sulaiman, Rokiah Hashim, leh Cheu Peng, Rajeev Pratap Singh

چکیده


Pycnoporus sanguineus is a white rot fungus that is known for selective and destructive wood bio delignification in tropical forests. This filamentous fungus is harvested on oil palm biomass (OPB) under solid substrate fermentation (SSF) and produces white-rot decay by generating ligninolytic enzyme Laccase (Lac). Laccase is produced at room temperature in the presence of Kirk’smedia supplemented with glucose, ammonium nitrate and corn steep liquor. The Lac enzymes are able to endure temperatures ranging from 10 to 700C and also the pH variation from 2.5-6. The synthesis of this enzyme, accountable for lignin degradation in oil palm biomass can further be exploited to degrade the unmanageable organic contaminants in the atmosphere. It is observed that selective lignin degradation was produced by P.sanguineus for time period of 4 weeks. The weight and component losses of oil palm trunk chips after decay were evaluated and analyzed by Scanning Electron Microscope. The maximum Laccase activity was 1007. 8 U/L on the 10th day of incubation. The present work is dedicated to assess the biodegradation of oil palm biomass and study for its pretreatment of enzyme activity

واژگان کلیدی


Fungal pretreatment; Lignocellulose; Pycnoporus sanguineus; Laccase

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