Developing New Antimicrobial Therapies: as a Candidate for the Treatment of Bacterial Infection with PER-1 gene Resistance to Antibiotics | ||
| Plant, Algae, and Environment | ||
| دوره 8، شماره 2، 2024، صفحه 1345-1354 اصل مقاله (437.57 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.48308/jpr.2024.234198.1059 | ||
| نویسندگان | ||
| Zuhoor Almousawi1؛ Neda Soleimani* 2؛ Gholam Abbas Kaydani3؛ Nasim Ebrazeh4 | ||
| 1Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Behishti University | ||
| 2Department of Microbiology and Microbial Biotechnology, Shahid Beheshti University, Tehran, Iran | ||
| 3Assistant Professor of Medical Virology Department of Laboratory Sciences, School of Allied Medical Sciences, Ahvaz Jundishapur University of Medical Sciences | ||
| 4Advanced Industries Research and Development Support Fund (airdfh), Trade Advanced Support Fund for Research and Development, Tehran, Iran | ||
| چکیده | ||
| The emergence of multidrug resistance in bacteria due to the overuse of antibiotics has become an important health concern in recent years, which requires the development of novel alternatives to fight against. Essential oils (EOs) are secondary metabolites that have different components and chemical compositions, which may provide promising solutions to the problem of the rising number of drug-resistant bacteria. This study aims to determine the efficacy of Eucalyptus and Geranium oil against Klebsiella pneumoniae, antibiotic-sensitive profile, and detect the molecular of the PER-1 gene by PCR method. In total 60 isolates of K. pneumoniae were collected from different hospitals in Ahvaz, (Golestan and Imam, Iran). Clevenger-type apparatus was used for oils extraction of hydro-distilled plants aerial, to determine the efficacy of essential oils. The agar well diffusion method and disc diffusion method were applied for five antibiotic discs to detect the profile of antibiotic sensitivity. Molecular detection of the PER-1 gene was done by PCR method. The results indicated that the oil extract of each plants inhibit the growth of K. pneumoniae with zones of inhibition ranging from 25 mm. Klebsiella pneumonia isolates were highly resistant to ceftazidime (83.3%), and more sensitive to Eucalyptus camaldulensis Dehnh Molecular detection of extended-spectrum β-lactamase bla PER-1 gene was recorded 12 (20%). The Geranium and Eucalyptus essential oil can be used as an alternative treatment for diseases caused by resistance to clinical isolation of K. pneumoniae. | ||
| کلیدواژهها | ||
| Klebsiella pneumonia؛ PER-1 gene؛ Essential oils | ||
| مراجع | ||
|
Ahmad MR and Ahmad K. (2023). Antimicrobial properties of some plant essential oils against two human pathogens. International Journal of Pharmaceutical Chemistry and Analysis. 9 (4): pp. 184–187. Doi: https://doi.org/10.18231/j.ijpca.2022.033.
Almas I, Innocent E, Machumi F, Kisinza W. (2021). Chemical composition of essential oils from Eucalyptus globulus and Eucalyptus maculata grown in Tanzania. Scientific African. 12: e00758. Doi: https://doi.org/10.1016/j.sciaf.2021.e00758.
Assaggaf HM, Mrabti HN, Rajab BS, Attar AA, Hamed M, Sheikh RA, Omari NE, Menyiy NE, Belmehdi O, Mahmud S, Alshahrani MM, Park MN, Kim P, Zengin G, Bouyahya A. (2022). Singular and combined effects of essential oil and honey of Eucalyptus globulus on anti-inflammatory, antioxidant, dermatoprotective, and antimicrobial properties: In vitro and In vivo findings. Molecules. 27 (16). Doi: https://doi.org/10.3390/molecules27165121.
Bigos M, Wasiela M, Kalemba D, Sienkiewicz M. (2012). Antimicrobial activity of geranium oil against clinical strains of Staphylococcus aureus. Molecules. 17 (9): 10276–10291. Doi:https://doi.org/10.3390/molecules170910276.
Castanheira MS and Bradford PA. (2021). Extended-spectrum β-lactamases: An update on their characteristics, epidemiology and detection. JAC-Antimicrobial Resistance. 3 (3). Doi: https://doi.org/10.1093/jacamr/dlab092.
Abbas FM. (2021). PER β-lactamase in Klebsiella pneumoniae resistant to carbapenem from Al-Hillah Teaching Hospital environment. 9 (3): pp: 128–132.
Dumlupinar B, Karatoprak GS, Demirci B, Akkol EK, -Sánchez ES. (2023). Antioxidant activity and chemical composition of Geranium Oil and Its synergistic potential against Pneumococci with various antibiotic combinations. Plants. 12 (17). Doi: https://doi.org/10.3390/plants12173080.
Fenghour H, Bouabida H, Dris D, Houhamdi M. (2021). Antibacterial effect of essential oils of two plants Eucalyptus camaldulensis and Artemisia herba alba on some bacterial strains. Biosystems Diversity. 29 (2): pp. 73–77. Doi: https://doi.org/10.15421/012110.
Galovičová L, Cimkova N, Schwarzová M, Vukic MD, Vukovic NL, Kowalczewski PL, Bakay L, Kluz MI, Puchalski C, Obradovic AD, Mati´ MM, Kaˇcániová M. (2023). Biological activity of Cupressus sempervirens essential oil. Plants. 12 (5). Doi: https://doi.org/10.3390/plants12051097.
Jafari SA, and Shariat A. (2020). Synergistic effects of silver nanoparticles with ethanolic extract of Eucalyptus globules on standard pathogenic bacteria in vitro. Tabari Biomedical Student Research Journal [Preprint]. (2). Doi: https://doi.org/10.18502/tbsrj.v2i3.4528.
Kadivarian S, Hosseinabadi S, Abiri R, Kooti S, Alvandi A. (2023). Frequency of extended-spectrum B-lactamase-producing genes associated with gram-negative bacteria isolated from infectious patients in Kermanshah (2019-2020). Iranian Journal of Medical Microbiology. 17 (1): 39–49. Available at: https://doi.org/10.30699/ijmm.17.1.39.
Khalaf ZZ, and Zahra LA. (2020). Evaluation of the activity of essential oil and hydrosol from eucalyptus camaldulensis against some bacterial species. Iraqi Journal of Science. 61 (6): 1282–1288. Doi: https://doi.org/10.24996/ijs.2020.61.6.5.
ŁysakowskaM, Sienkiewicz M, Banaszek K, Sokołowski J. (2015). The sensitivity of endodontic Enterococcus spp. strains to Geranium essential oil. Molecules. 20 (12): pp. 22881–22889. Doi: https://doi.org/10.3390/molecules201219888.
Mehani M and Ladjel S. (2012). Antimicrobial effect of essential oils of the Plant Eucalyptus camaldulensis on some pathogenic bacteria. International Journal of Environmental Science and Development. (January 2012), pp. 86–88. Doi: https://doi.org/10.7763/ijesd.2012.v3.193.
Odari R, and Dawadi P. (2022). Prevalence of multidrug-resistant Klebsiella pneumoniae clinical isolates in Nepal. Journal of Tropical Medicine.. Doi: https://doi.org/10.1155/2022/5309350.
Oliveira R, Castro J, Silva S, Oliveira H, Saavedra MJ, Azevedo NF, Almeida C. (2022). Exploring the antibiotic resistance profile of clinical Klebsiella pneumoniae isolates in Portugal. Antibiotics. 11 (11): 1–14. Doi: https://doi.org/10.3390/antibiotics11111613.
Opi K, Dhinsa K, Tripath AM, Saha S. (2023). To evaluate and compare the antimicrobial efficacy of various disinfecting agents on K-file against gram-positive and gram-negative bacteria of endodontic origin: An In vitro study. International Journal of Clinical Pediatric Dentistry. 16 (S2) S161–S167. Doi: https://doi.org/10.5005/jp-journals-10005-2646.
Remya PA, Shanthi M, Sekar U. (2019). Characterization of virulence genes associated with pathogenicity in Klebsiella pneumoniae. Indian Journal of Medical Microbiology. 37 (2): 210–218. Doi: https://doi.org/10.4103/ijmm.IJMM_19_157.
Shaik G, Sujatha N, Mehar SK. (2014). Medicinal plants as source of antibacterial agents to counter Klebsiella pneumoniae. Journal of Applied Pharmaceutical Science. 4 (1): 135–147. Doi: https://doi.org/10.7324/JAPS.2014.40123.
Srivastava A, Kumar V, Agarwal V. (2022). Antimicrobial activity of some essential oils against Pseudomonas aeruginosa. Emerging Trends in Biotechnology (Biosanagam 2022). 27-34. Doi: https://doi.org/10.2991/978-94-6463-020-6.
Tran HD, Nguyen NC, Nguyen HD, Nguyen HTD, Nguyen TTT, Nguyen PH, Tran HG. (2023). The situation of antibiotic resistance in Klebsiella Pneumoniae and carbapenemase-producing Klebsiella Pneumoniae in Vietnam: A cross-sectional study. Journal of Health Science and Medical Research. p. 2023964. Doi: https://doi.org/10.31584/jhsmr.2023964.
Zhang Z, Morgan CE, Bonomo RA, YUEW. (2023). Cryo-EM structures of the Klebsiella pneumoniae AcrB multidrug efflux pump. mBio. 14 (3). Doi: https://doi.org/10.1128/mbio.00659-23. | ||
|
آمار تعداد مشاهده مقاله: 137 تعداد دریافت فایل اصل مقاله: 143 |
||